鬼筆環(huán)肽是一種雙環(huán)七肽毒素，特異性結(jié)合在F-肌動蛋白亞基之間的界面上，將相鄰的亞基鎖定在一起。鬼筆環(huán)肽與肌動蛋白絲的結(jié)合比與肌動蛋白單體的結(jié)合要緊密得多，從而導(dǎo)致肌動蛋白亞基從絲端解離的速率常數(shù)降低，因此通過防止絲解聚而基本穩(wěn)定了肌動蛋白絲，終，發(fā)現(xiàn)鬼筆環(huán)肽抑制F-肌動蛋白的ATP水解活性。

鬼筆環(huán)肽的性質(zhì)是研究F-肌動蛋白在細(xì)胞中分布的有用工具，方法是用熒光類似物標(biāo)記鬼筆環(huán)肽并將其用于染色肌動蛋白絲以進(jìn)行光學(xué)顯微鏡檢查。鬼筆環(huán)肽的熒光衍生物已被證明在尋找活細(xì)胞或固定細(xì)胞中的肌動蛋白絲以及體外觀察單個肌動蛋白絲方面非常有用。熒光鬼筆環(huán)肽衍生物已被用作高分辨率研究肌動蛋白網(wǎng)絡(luò)的重要工具。AAT Bioquest可以提供多色成像應(yīng)用的鬼筆環(huán)肽。

參考文獻(xiàn)

HMGB1 released by irradiated tumor cells promotes living tumor cell proliferation via paracrine effect
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DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
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公司簡介
西安百螢生物科技有限公司是一家專注從事生物、化學(xué)試劑及其試劑盒的高新技術(shù)企業(yè)；主要以熒光探針，分子探針，核酸/蛋白質(zhì)標(biāo)記、細(xì)胞檢測等技術(shù)和產(chǎn)品為研發(fā)核心，產(chǎn)品應(yīng)用于基因合成、細(xì)胞檢測、活體示蹤，抗體標(biāo)記、新藥篩選等領(lǐng)域。